• Users Online: 175
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Year : 2019  |  Volume : 24  |  Issue : 2  |  Page : 78-81

Evaluation of nitrate reductase assay on Lowenstein–Jensen medium for drug susceptibility testing of Mycobacterium tuberculosis

1 Department of Microbiology, Universal Diagnostic Laboratory, Pusad, Yavatmal District, Wardha, Maharashtra, India
2 Department of Microbiology, Mahatma Gandhi Institute of Medical Sciences, Wardha, Maharashtra, India

Correspondence Address:
Dr. Rahul Narang
Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, Maharashtra
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jmgims.jmgims_59_16

Rights and Permissions

Introduction: Multidrug-resistant tuberculosis is of increasing public health concern and a rapid and inexpensive drug susceptibility test is needed in resource limited settings. Aim: This study aims to compare the efficacy of drug susceptibility testing (DST) of Mycobacterium tuberculosis using nitrate reductase assay (NRA) on solid Lowenstein–Jensen (LJ) medium with proportion method on LJ medium as reference standard. Materials and Methods: From November 2011 to July 2012, 100 isolates of M. tuberculosis were tested for susceptibility to first-line drugs on LJ medium by proportion method and NRA using LJ with potassium nitrate. Results: For various drugs, the sensitivity and specificity by NRA on solid medium were as follows: streptomycin 96.6% and 98.5%; isoniazid 96.87% and 97%; rifampicin 96.3% and 96%; and ethambutol 78.9% and 91.4%, respectively. The concordance for rifampicin and isoniazid between NRA method and LJ proportion method was found to be >95%. Conclusion: NRA was found to be a rapid and sensitive method for DST of M. tuberculosis.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded286    
    Comments [Add]    

Recommend this journal